The Mayor of London criticised Mrs May’s delivery style after her keynote speech on Wednesday at the annual party gathering.
The Prime Minister was widely applauded for her confident showing during the speech, which saw her arrive on stage dancing to Abba’s Dancing Queen – a nod to her ridiculed moves during a visit to Africa.
But during a speech on artificial intelligence, Mr Khan – currently under fire for London’s soaring violence rate – told technology experts at Thursday’s Business Insider UK Tech 100 party he was not impressed with what critics call the Maybot.
Mr Khan said: “We’ve got this amazing technology now where an entire prime minister’s speech can be delivered by a robot and nobody notices.”
The Prime Minister has been dubbed the Maybot for her lack of charisma.
And her rigid dancing style, unveiled during a show from Kenya scouts, did nothing to stop the teasing.
Despite widespread ridicule at the time, the Maybot took centre stage again during Wednesday’s speech.
During Mr Khan’s UK Tech 100 speech, he also called on tech companies Facebook and Twitter to cooperate more closely with lawmakers.
Mr Khan told Business Insider: “It is a reality that we have seen elections influenced by technology.
“It is a reality that we’ve seen people radicalised by technology.
“It is a reality we’ve seen the promotion of hatred, particularly against women and minorities.
“The way to address those concerns isn’t to destroy that innovation, it’s for the tech sector to work with politicians to try and grapple with some of these problems.”
Britain appears set to introduce new regulations to tech giants on subjects such as illegal online hate speech.
Mr Khan also said he wanted London to become the tech capital of the world.
He added: “London is the tech capital of Europe and my mission is for us to be the tech capital of the world.
“Not just because I’m competitive, but because I can see a difference tech makes to reshaping our society, reshaping our economy, and reshaping our culture.”