The billionaire businessman stormed to victory in the ballot with 29.6 per cent of the ballot for his ANO party.
Speaking last night, the populist leader said the EU needs to “stop talking about a two-speed Europe” and reflect on the reasons for Britain voting Leave.
Mr Babis said in his victory speech at ANO headquarters: “The European Union needs to reflect to think about why Britain has chosen to leave it and to stop talking about a two-speed Europe.
“I am happy that Czech citizens did not believe the disinformation campaign against us and expressed their trust in us.”
“We are a democratic movement, we are a pro-European and pro-NATO party, and I do not understand why somebody labels us as threat to democracy.”
French leader Emmanuel Macron has previously outlined his plans for sweeping European Union reforms, which include a strengthening of the economic union between key member states, like France and Germany.
And plans for a “two-speed Europe”, where western, original members of the bloc take up increased integration, while newer member states to the east are left to catch up, have also been mooted.
Exit polls show Mr Babiš’s nearest rival, the SPD party, earned 11.6 per cent of the vote.
But even with such a convincing win, the former Finance Minister will need to form a coalition in order to govern.
Mr Babiš said he is willing to negotiate with all eight of his rival parties, adding he hopes a government can be formed quickly.
However the head of the right-wing SPD has reportedly ruled out any coalition talks.
Mr Babiš insisted his ANO party was “pro-European”, despite being critical of the EU’s immigration policy and the euro currency on the campaign trail.
Ninety per cent of the votes have been counted so far, and exit polls place the centre-right Civic Democracy Party third with 10.8 per cent of the vote.
The Czech Pirate Party looks to be the country’s fourth biggest party with 10.29 per cent, with the Communists closely behind at 8.08 per cent.
The results show that eight parties in total have secured the minimum five per cent threshold to enter the Czech parliament.